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简介:
内参抗体包括beta-actin、beta-Tubulin、GAPDH等,可在进行Western blot实验时检测目的产物、校正上样误差,也可作为空白对照,检测蛋白转膜是否*、整个显色或者发光体系是否正常,是Western blot 实验必选的实验抗体。
内参蛋白的选择:
常用的细胞总蛋白质内参有GAPDH(glyceraldehyde-3-phosphate dehydrogenase)和细胞骨架蛋白beta-actin或beta-tubulin。对于一些植物的样本,则需要特别的植物Actin蛋白作为内参;同样的,如果提取的样本是细胞核蛋白,那么PCNA或者组蛋白H3都是被经常使用的;而针对一些研究线粒体的客户,那么Cox IV则被较多的文献引用作为线粒体总蛋白的内部参考。博蕾德生物拥有足够的生产能力和完善的质量管理体系,自产的内参抗体,种类齐全,性能稳定,质量可靠,可长期稳定储存
内参蛋白抗体产品信息
内参抗体 | 货号 | 名称 | 反应种属 | 应用 |
β-actin | BL041F | β-actin Mouse MAb(5B7) | H, R, M, Mk, Dg, Ch, Hm, Rb | WB、 IHC、IF |
BL045F | β-actin Mouse MAb (A02) | H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh | WB 、IHC、 IF | |
BL166F | β-actin Mouse MAb (Mix-mA™) | H, R, M, Mk, Dg, Ch, Hm, Rb | WB 、IHC、 IF | |
BL161F | β-actin Mouse MAb (M5) | H, R, M, Mk, Dg | WB | |
BL051F | β-actin Rabbit PAb Antibody | H、M、R、Rb、Ch、Mk、Sh、Xenopus | WB 、IHC、 IF | |
BL149F | β-actin Mouse MAb (Zebrafish Specific) | Zebrafish | WB 、IHC、 IF | |
BL056F | β-actin Mouse MAb (5B7)-HRP conjugated | H, R, M, Mk, Dg, Ch, Hm, Rb | WB | |
GAPDH | BL042F | GAPDH Mouse MAb (2B8) | H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh | WB 、IHC、 IF |
BL052F | GAPDH Rabbit PAb | H、M、R、Rb、Ch、Mk、Sh、Xenopus | WB 、IHC、 IF | |
BL057F | GAPDH Mouse MAb (2B8)-HRP conjugated | H, R, M, Mk, Dg, C, Hm, Rb, Pg, Sh | WB | |
BL141F | GAPDH Rabbit PAb(Zebrafish Specific) | Zebrafish | WB、IHC | |
α-tubulin | BL048F | α-tubulin Mouse Monoclonal Antibody(8F11) | H, R, M | WB、IHC |
BL054F | α-tubulin Rabbit Polyclonal Antibody | H、M、R | WB、IHC | |
BL143F | α-tubulin Mouse Monoclonal Antibody(Zebrafish Specific) | Zebrafish | WB | |
β-Tubulin | BL043F | β-Tubulin Mouse MAb (5G3) | H, R, M, Mk, Dg, Ch, Hm, Rb,Sh | WB IHC IF |
BL050F | β I tubulin Mouse MAb (3F7) | H, R, M | WB | |
BL142F | β-tubulin Mouse MAb (M7) | H,M,R | WB、IF | |
BL147F | β-Tubulin Mouse MAb(Zebrafish Specific) | Zebrafish | WB IHC | |
BL163F | β I tubulin Mouse MAb (Zebrafish Specific) | Zebrafish | WB | |
BL167F | β-tubulin Mouse MAb (Mix-mA™) | H, R, M, Mk, Dg, Ch, Hm, Rb | WB IHC IF IP | |
BL191F | β II tubulin Mouse MAb (Mix-mA™) (Rodent Preferred) | M,R,(H) | WB | |
植物Actin | BL047F | Plant actin Mouse MAb (Q30) | Various Plants | WB |
BL150F | Plant actin Mouse MAb (2E3) | Various Plants | WB | |
BL053F | Plant-actin Rabbit PAb | Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgare | WB | |
BL187F | Plant actin Mouse Monoclonal Antibody(Mix-mA™) | Various Plants | WB | |
PCNA | BL044F | PCNA Mouse MAb (12D10) | H, R, M | WB IHC IF |
COX IV | BL046F | COX IV Mouse MAb (BL100A-6C8) | H, R, M | WB IF |
Lamin B1 | BL049F | Lamin B1 Mouse Monoclonal Antibody(7C11) | H, R, M | WB、IP |
Histone H3 | BL055F | Histone H3 Mouse Monoclonal Antibody(1G1) | H、M、R | WB、IHC、IF、IP |
BL188F | Histone H3 Mouse Monoclonal Antibody(Mix-mA™) | H,M,R | WB、IP | |
BL190F | Histone H3 Mouse Monoclonal Antibody(8F7) | H,M,R | WB | |
BL148F | Histone H3 Mouse Monoclonal Antibody(Zebrafish Specific) | Zebrafish | WB |